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Sasidharan, T. O.
- Effects of Different Adjuvants on the Growth and Sporulation of the Entomopathogenic Fungus, Metarhizium anisopliae (METSCH)
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Authors
Affiliations
1 Department of Entomology, Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Srirampura, Jakkur P.O., Bengaluru-560064, IN
1 Department of Entomology, Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Srirampura, Jakkur P.O., Bengaluru-560064, IN
Source
Indian Forester, Vol 140, No 1 (2014), Pagination: 93-97Abstract
Assessment of the effect of formulation components on viability of fungal conidia is imperative prior to their use in formulations. A range of adjuvants were evaluated for their effect on growth and sporulation of three Metarhizium isolates to identify an appropriate adjuvant for developing a liquid formulation. Oils such as neem oil, pongam oil, eucalyptus oil, SG oil, coconut oil and diesel, each at 0.1, 0.5 and 1.0% and mineral oil at 1,2,3,4&5% concentrations and surfactants such as glycerol at 0.1, 0.2, 0.5, 0.8&1.0% and tween-80 at 0.01, 0.02, 0.05&0.08% concentrations were tested to find out the most appropriate adjuvant and its optimum concentration required for making liquid formulations of Metarhizium. Even though biomass and sporulation in mineral oil formulation was slightly superior to pongam oil formulation, the insecticidal activity of pongam oil endows an added advantage for its use in formulations for controlling insect pests. The observations on biomass produced after 7 days of treatment with surfactants revealed that treatments with tween-80 at 0.08 and 0.05% and glycerol at 1.0 and 0.8% were significantly superior to untreated control.Keywords
Metarhizium, Anisopliae, Entomopathogens, Formulation, Oil , Surfactants.
References
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- Metarhizium majus and Metarhizium robertsii Show Enhanced Activity against the Coleopteran Pests Holotricha serrata and Oryctes rhinoceros
Abstract Views :284 |
PDF Views:140
Authors
Affiliations
1 ICAR-National Bureau of Agricultural Insects Resources, Bangalore – 560024, Karnataka, IN
2 Institute of Wood Science and Technology, Bangalore – 560 003, Karnataka, IN
3 Ashoka Trust for Research in Ecology and the Environment (ATREE), Bangalore – 560064, Karnataka, IN
1 ICAR-National Bureau of Agricultural Insects Resources, Bangalore – 560024, Karnataka, IN
2 Institute of Wood Science and Technology, Bangalore – 560 003, Karnataka, IN
3 Ashoka Trust for Research in Ecology and the Environment (ATREE), Bangalore – 560064, Karnataka, IN
Source
Journal of Biological Control, Vol 31, No 3 (2017), Pagination: 135-145Abstract
Studies were conducted to systematically isolate Metarhizium isolates from the insect cadavers and soils of South India. Morphological and PCR amplified sequences of 5.8S ITS regions and RNA polymerase II largest subunit (RPB1) gene regions were used to identify the isolates at species level. Eight Metarhizium isolates were isolated and initially identified by morphological and microscopic studies. Further identification was confirmed through 5.8SrRNA ITS and RPB1 analysis. They were identified as three isolates of M. robertsii J.F. Bisch., Rehner & Humber sp. nov. (ArMz3R, ArMz3S and ArMz6W), one isolate of M. majus (J.R. Johnst.) J.F. Bisch., Rehner & Humber (VjMz1W) and four isolates of M. anisopliae (WnMz1S, NlMz2S, BgMz2S and DhMz4R). Topical conidial suspensions (TCS) and powder based formulations (PBF) of the eight indigenous isolates of Metarhizium spp. that were isolated from insect cadavers and soils of South India were tested against coleopteran pests Holotricha serrata L. and Oryctes rhinoceros L. that cause serious damage to sugarcane and palm trees respectively. Against H. serrata TCS of M. robertsii (ArMz6W) was the most effective with an LC50 of 6.893×105 cfu/ml and caused 100% mortality against the 3rd instar larvae in 5 days; PBF elicited an LC50 of 7.502×105 cfu/ml with 96% mortality in 10 days. Against O. rhinoceros TCS (LC50 of 9.75×105 cfu/ml) of M. majus (VjMz1W) caused 90% mortality in 7 days and the PBF (LC50 of 9.57×105 cfu/ml) caused 86% mortality in 14 days. The results establish that M. robertsii is highly effective against H. serrata and against O. rhinoceros, M. majus was the most effective. The TCS formulations of these two strains can be readily deployed for field applications.Keywords
Holotricha serrata, Metarhizium spp., Oryctes rhinoceros, Powder Based Formulation (PBF), Topical Conidial Suspension (TCS).Full Text
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